Table 1 Study variables and data collection questionnaires to be used in the study

Last month of gestation

General information

Name, address, phone number, maternal ID, maternal date of birth, health social scheme.

Maternal socioeconomic and demographic information

Marital status, socioeconomic level, type of housing, maternal occupation, monthly income, educational level, maternal leave, income assigned to food purchase, access to tap water, person in charge to infant, breastfeeding family support.

Pregnancy information

Maternal smoking, obstetric background, gestational age, probable date of birth, supplement and vitamins intakes, laboratory reports, medical history.

Paternal information

Educational level, monthly income, weight and height (self-reported), medical history.

Intention to breastfeed

Five questions about intention to breastfeed or give formula until 6 months postpartum.

Maternal anthropometry

Pregestational weight (self-reported), weight, height and MUAC measurements.

Breast examination

Nipple and breast self-perception.

Counselling assessment

Detected problems and concerted solutions.

IPAQ SV

Physical activity registry: type of activity, time (minutes per day), number of days per week.

EPDS

Ten questions to evaluate maternal emotional status.

Maternal 24-h dietary recall

Five steps 24HR: fast list, frequently forgotten foods, meals and time, food consumption in the last 24 h within detailed registry and standardized recipes.

Semi-quantitative food frequency intake

Diary, weekly or monthly intake of 107 foods included in 8 food groups.

24 h postpartum

Maternal anthropometry

Information about delivery and newborn

Weight and MUAC.

Gender, date of birth, type of delivery, complications, gestational birth age, APGAR at 5 min, time of birth.

Newborn anthropometry

Birth weight, length, head circumference, MUAC.

Breastfeeding information

Time of skin to skin contact, duration of skin to skin contact, breastfeeding during the first hour after delivery, breastfeeding difficulties.

Infant 24-h recall

Time, place, infant feeding in the last 24 h (including breastfeeding, infant formula or other type of foods given to the baby).

Breast milk intake

Number of breastfeeds per day and night, time per each breastfeed, appetite (mother’s perception).

Breast latching

Observation of latching, swallowing, type of nipple, maternal comfort perception and breastfeeding position.

Counselling registry at 24 h, 3th day, 7th day and 14th day

Detected problems and agreed solutions, success of breastfeeding counselling regarding the last meeting’s concerted solutions.

1 month postpartum

Maternal anthropometry

Weight and MUAC.

Infant anthropometry

Weight, length, head circumference, MUAC.

Counselling registry

Detected problems and concerted solutions, success of breastfeeding counselling regarding the last meeting’s concerted solutions.

IPAQ SV

Physical activity registry: type of activity, time (minutes per day), number of days per week.

EPDS

Ten questions to evaluate maternal emotional status.

Maternal 24-h recall

Five steps 24HR: fast list, frequently forgotten foods, meals and time, food consumption in the last 24 h within detailed registry and standardized recipes.

Semi-quantitative food frequency intake

Diary, weekly or monthly intake of 107 foods, included in 8 food groups.

Infant 24-h recall

Time, place, infant feeding in the last 24 h (including breastfeeding, infant formula or other type of foods given to the baby).

Breast milk intake

Number of breastfeeds per day and night, time per each breastfeeds, appetite (mother’s perception).

Breast milk macronutrient content

Protein, lactose and fat content in human milk

Prolactin in maternal serum

Concentration of prolactin hormone in maternal serum, 2 h after maternal wake up

3 months postpartum

Maternal anthropometry

Weight and MUAC.

Infant anthropometry

Weight, length, head circumference, MUAC.

Breast examination

Nipple and breast observation.

Counselling registry

Detected problems and concerted solutions, applicability of last meeting’s concerted solution

Infant 24-h recall

Time, place, type of food.

Breast milk intake

Number of breastfeeds per day and night, time per each breastfeed, appetite (mother’s perception).

Macronutrients content in breast milk

Protein, lactose and fat content in human milk.

Prolactin in maternal serum

Concentration of prolactin hormone in maternal serum, 2 h after maternal wake up.

4 months postpartum

Maternal anthropometry

Weight and MUAC.

Infant anthropometry

Weight, length, head circumference, MUAC.

Breast examination

Nipple and breast observation.

Assessment of breastfeeding counselling

Successful of new approach in EBF counselling with 5 questions to assess concerted solutions.

IPAQ SV

Physical activity registry: type of activity, time (minutes per day), number of days per week.

Maternal 24-h recall

Five steps 24HR: fast list, frequently forgotten foods, meals and time, food consumption in the last 24 h within detailed registry and standardized recipes.

Semi-quantitative food frequency intake

Diary, weekly or monthly intake of 107 foods, included in 8 food groups.

Infant 24-h recall

Time, place, infant feeding in the last 24 h (including breastfeeding, infant formula or other type of foods given to the baby).

Breast milk intake

Number of breastfeeds per day and night, time per each breastfeed, appetite (mother’s perception).